Biocytin-filled
WebJun 13, 2007 · To measure the length of neuronal dendrite covered by an astrocyte, confocal z-series containing the biocytin-filled neuron and EGFP-positive astrocytes were studied using Volocity software. Single astrocytes were identified as mentioned previously. Of the nine layer 2/3 neurons that were successfully loaded with and stained for … WebAccurate 3D reconstruction of neurons is vital for applications linking anatomy and physiology. Reconstructions are typically created using Neurolucida after biocytin histology (BH). An alternative inexpensive and fast method is to use freeware such as Neuromantic to reconstruct from fluorescence imaging (FI) stacks acquired using 2-photon laser …
Biocytin-filled
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WebJun 17, 2024 · Reconstructions were based on a digitized, brightfield image stack of single, biocytin-filled neurons using semi-automated and manual tools in the software Vaa3D 15 (Fig. 1a). We chose neurons for ... WebJan 1, 2024 · Biocytin-filled neurons were fixed in 4% PFA in 0.1 M PB at 4°C for at least 24 h. Endogenous peroxidase activity was quenched with a 3% H 2 O 2 solution for 15 min. Sections were incubated overnight at …
WebIn more details, a biocytin (C 16 H 28 N 4 O 4 S) self-assembled monolayer is adsorbed onto the base of a CaF 2 prism closing a Teflon cell. The latter is then filled with a … WebAug 19, 2024 · To analyze the dendritic morphology of biocytin-filled human neurons, we imported the confocal images into IMARIS (version 9.2, Bitplane) neuronal reconstruction …
WebSep 29, 2014 · Importantly, however, reconstruction of biocytin-filled PTEN KO cells occasionally revealed multiple axon collaterals projecting into CA3 (2 of 6 neurons), while all control cells examined had only a single collateral in this region (n=5). Mossy fiber sprouting, in which granule cell axons project back into the molecular layer, was also observed. WebThis biocytin filled neuron is a fast-spiking, parvalbumin-expressing GABAergic basket cell in the CA1 region of the mouse hippocampus. Dendrites (blue) of this cell type …
WebAug 1, 2011 · HEGCs and normotopic granule cells were filled with biocytin during whole-cell patch clamp recording in hippocampal slices from pilocarpine-treated rats. The apical dendrite of 86% of the biocytin-labeled HEGCs extended to the outer edge of the dentate molecular layer. The total length and branching of HEGC apical dendrites that penetrated …
WebNov 9, 2015 · The tracer was detected using streptavidin-conjugated horseradish peroxidase, made visible by precipitation of diaminobenzidine (DAB) for light microscopy. After whole-cell patch-clamp recording, tissue with biocytin-filled type II afferents was fixed in 4% (vol/vol) PFA overnight at 4 °C, and stored in PBS for further processing within 2 wk. sunflower auto auctionWebApr 10, 2024 · A biocytin-filled VMHvl cell (c2, right). d , Example light-evoked EPSC (oEPSC) and IPSC (oIPSC) ( d1 ). The percentage of VMHvl Esr1 cells showing oIPSC only or both oIPSC and oEPSC ( d2 ). palmerston flower shopWebRight , the number of dye-coupled astrocytes was measured in confocal images of biocytin-filled, streptavidin-AF488-labeled cells, and was similar during the L/D-phases. Article Snippet: 3D z-stacks of biocytin filled CA1 pyramidal neurons were acquired using a Zeiss LSM710 confocal microscope and saved as czi files. palmerston forts society publicationsWebThe invention generally relates to compositions and methods for transporting substances across mucosal barriers. The invention also relates to methods of making and using such substances. sunflower apts wyoming miWebNov 20, 2024 · The protocol outlined here describes how biocytin-filled cells recorded in the CA2 region of the hippocampus are recovered and then reconstructed in 3D. … sunflower auction jasper flWebBiocytin is a chemical compound that is an amide formed from the vitamin biotin and the amino acid L -lysine. As an intermediate in the metabolism of biotin, biocytin occurs … sunflower arizonaWebSep 9, 2024 · To reconstruct the full morphology of biocytin filled or GFP-labeled neurons , imaging was performed on a Leica TCS SP8 confocal laser-scanning microscope (Bensheim, Germany) using a 40x/1.3NA oil-immersion objective. Z-stack tile scans of the whole culture slice were acquired. The Cy3 fluorescence was excited at 548 nm using a … sunflower at cover