WebApr 14, 2024 · Later, cells were incubated with C11-BODIPY (2.5 μM) (ThermoFisher, D3861) in fresh medium and incubated in the dark 1 h at 37 °C. Cells were then harvested while protected from light, suspended ... WebBODIPY-C11 staining was performed according to the manufacturer’s protocol. The cells were seeded in a 35-mm-diameter dish. Culture medium was replaced with 2 ml medium containing 5 µM of BODIPY-C11 (Cat. No. D3861; Invitrogen) after 48 h and the culture was returned to the cell culture incubator for 30 min.

Tagitinin C induces ferroptosis through PERK-Nrf2-HO-1 signaling ...

WebBrand: Invitrogen™ D3861 Additional Details : Weight : 0.00525kg ... In reduced state, the excitation and emission maxima of BODIPY™ 581/591 undecanoic acid is 581/591 nm; … WebMay 9, 2024 · An amount of 10 μM BODIPY-581/591 C11 (D3861, Thermo Fisher Scientific) was added to the media and incubated for 30 min at 37 °C, 5% CO 2. The ... know your itr status

Inhibition of the PIN1-NRF2/GPX4 axis imparts sensitivity to.

WebFeb 10, 2024 · Afterwards, 2 μl of 10 mM BODIPY-C11 (D3861, Thermo Fisher Scientific) was added and incubated at 37°C for 30 min. All cells, including some detached and floating cells, were harvested and washed twice with PBS. Thereafter, the supernatant was separated, and the cell pellet was resuspended with 500 µl PBS. WebLipid Peroxidation Sensor BODIPY ® 581/591 (Cat: D3861, Thermo Fisher) was used to detect reactive oxygen species (ROS) in cells and membranes according to the manufacture introduction. After HPSC cells were treated as indicated, C11-BODIPY was added (2.5 μL/mL) and incubated for 10 min. Excess dye was removed by washing the cells twice … WebFeb 27, 2007 · BODIPY ® 581/591 C 11 (D3861, Molecular Probes) was added to 2 × 10 6 spermatozoa at a final concentration of 5 µM and allowed to incubate for 30 min at 37°C. The cells were washed twice at 650 g for 5 min and then treated with either vehicle control or promoter (80 µM ferrous sulphate unless specified otherwise) in BWW/PVA for 15 or … redbird ahip