Fluorescein diphosphate
WebJul 2, 2016 · A highly sensitive and continuous protein tyrosine phosphatase (PTPase) assay using 3,6-fluorescein diphosphate (FDP) is described. Leukocyte phosphatase … WebJul 1, 2003 · CD45 is captured from cell lysates using anti-CD45 monoclonal antibody coated onto a 96-well plate, and the phosphatase activity is measured using the substrate fluorescein diphosphate (FDP).
Fluorescein diphosphate
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WebJun 10, 1996 · A new fluorogenic phosphatase assay requiring no prelabeling has been developed to quantitate cell number, which can thus serve as the basis for quantitating cell adhesion or migration. The assay uses the non-fluorescent substrate 3,6-fluorescein diphosphate (FDP) whose dephosphorylation generates fluorescein. WebNov 5, 2009 · Although a loss-of-function mutation has been identified in familial Parkinson’s disease PARK7, the wild-type of DJ-1 is known to act as an oxidative stress sensor in neuronal cells. Recently, we identified UCP0045037 as a compound that bound to the reduced form of DJ-1 by in silico virtual screening. In this study, we determined the …
WebFeb 1, 1999 · PDF A highly sensitive and continuous protein tyrosine phosphatase (PTPase) assay using 3,6-fluorescein diphosphate (FDP) is described. Leukocyte... … WebAug 1, 2024 · The TPE-Zr4+ aggregates also retained the phosphatase-like activity, which can catalyze the fluorescent substrate fluorescein diphosphate (FDP) to produce green …
WebFluorescein diphosphate is a colorless and nonfluorescent substrate for alkaline phosphatases, sequential alkaline phosphatase mediated hydrolysis of its two phosphate … WebJun 12, 2024 · Non-fluorescent fluorescein diphosphate (tetra-ammonium salt, Insight Biotechnology) was used as the enzyme substrate and encapsulated in the water phase (100 μl, 1 mg ml −1, carbonate buffer ...
WebThis addition resulted in the release and diffusion of ALP-linked anti-human IgG into the bulk solution. This event led to a 20-min single-step sandwich immunoreaction at the inner wall of capillary; the reaction was detected through the reaction with fluorescein diphosphate (FDP) which generated a fluorescent product, fluorescein.
WebAug 6, 2024 · The first lock inhibits the main activity of fluorescein by phosphating. The second lock almost completely deactivates fluorescein by forming coordination … hm lahjakortti verkkokauppaWebActivity assays for tyrosine phosphatases are based on the hydrolysis of a arylphosphate moiety from a synthetic substrate yielding a spectroscopically active product. Many different substrates can be used for these assays with p-nitrophenyl phosphate (pNPP), fluorescein diphosphate (FDP), and 6,8-difluoro-4-methylumbellyferyl phosphate (DiFMUP ... hm kynttilätWebFeb 2, 2024 · A simple and cost-effective fluorescence immunoassay for the sensitive quantitation of disease biomarker α-fetoprotein (AFP) has been developed based … h m lahjakorttiWebMay 17, 1999 · Fluorescein diphosphate (FDP, 1) has been widely used for the detection of aikaiine phosphatase; the substrate is readily hydrolyzed by the enzyme, and after … hmlai storeWeb3,6-Fluorescein diphosphate C20H14O11P2 CID 131824 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological ... h&m laineWebOct 21, 2014 · Plant cell cultures as valuable tools for the production of specific metabolites can be greatly improved by the application of elicitors including cyclodextrins (CDs) for enhancing the yields of the desired plant compounds. Here the effects of 2,6-dimethyl-β-cyclodextrins (DIMEB) on the production of carotenoids and quinones from Artemisia … hm kynsilakkaWebApr 12, 1999 · In this series, fluorescein monosulfate monophosphate (FMSP) was the best substrate observed. Since FMSP showed large increases in both absorption and … hm lahti oy