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Lanthanide binding peptide

Tīmeklis2007. gada 6. jūn. · PMID: 17497862 DOI: 10.1021/ja070480v Abstract Lanthanide … Tīmeklis2024. gada 7. dec. · Brichtová, E.; Hudecová, J.; Vršková, N.; Šebestík, J.; Bouř, P.; …

Lanthanide-binding tags as versatile protein coexpression probes

Tīmeklis2016. gada 3. marts · LBTs have been broadly used as REE-binding fusion proteins for biological applications, including protein purification, biosensing, NMR spectroscopy, and X-ray crystallography.29–32The short peptide-based nature of LBTs and high REE binding affinity make them ideal for cell surface display to increase specific REE … TīmeklisThe separation of lanthanides from minor actinides such as americium and curium is an important step during the recycling process in the treatment of nuclear waste. However, the similar chemistry and ionic size of lanthanide and actinide ions make the separation challenging. Here, we report that a peptide-based reagent can selectively bind … top urologist in pune https://artworksvideo.com

Binding of Lanthanide Complexes to Histidine-Containing …

Tīmeklis2007. gada 12. maijs · A double-lanthanide-binding tag (dLBT), a small peptide sequence engineered to bind two lanthanide ions (e.g., Tb 3+) with high affinity, was used to solve the phase problem for the structure determination of ubiquitin by the single-wavelength anomalous diffraction (SAD) method. Tīmeklis2010. gada 1. apr. · Alternatively, peptide-based lanthanide-ion binding tags (LBTs) may be incorporated into proteins by standard molecular biology techniques, thereby avoiding steps that may be necessary for optimizing site-selective chemical modification [8].In this case, native protein sequences such as the Ca 2+-binding EF-hand … TīmeklisThe incorporation of unnatural chelating amino acids in short peptide sequences leads to lanthanide-binding peptides with a higher stability than sequences built exclusively from natural residues. In particular, the hexadentate peptide P22, which incorporates two unnatural amino acids Ada2 with aminodiacetate chelating arms, showed … top urologist in stuart florida

Rapid Combinatorial Screening of Peptide Libraries for the …

Category:Lanthanide-binding proteins could unlock new sources of valuable …

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Lanthanide binding peptide

Rationally designed mineralization for selective recovery of ... - Nature

TīmeklisThe peptide with the strongest binding affinity was the tetra-peptide with alternating … TīmeklisThe lanthanide binding site was rationally designed based around the coordination of Asp and Asn side chains. Asp was preferred to Glu because initial molecular dynamics modelling of the binding site suggested appropriate Ln–O …

Lanthanide binding peptide

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Tīmeklis2024. gada 13. apr. · In addition, since the Cu-binding peptide was fused with GFP, the binding between the peptide and Cu decreased the green fluorescence of GFP. Thus, the system could be also used as a biosensor for Cu. 4.1.5 Ni bioremediation. B. subtilis spore surface display has been used to develop protein-coated spores to adsorb Ni … Tīmeklis2013. gada 1. jūn. · Lanthanide-binding peptides are very attractive for the design …

TīmeklisA novel, compact, tunable protein-based lanthanide-binding motif was developed … Tīmeklis2003. gada 4. apr. · Lanthanide-binding tags (LBTs) are expressible, multitasking …

Tīmeklis2005. gada 21. dec. · A small, versatile and multifaceted co-expression tag would therefore be extremely useful to protein scientists: Lanthanide-Binding Tags (LBTs) fulfill such criteria. These tags are remarkably short peptide sequences (15 to 20 residues) comprising only the encoded amino acids; post-expression addition of a … Tīmeklis2004. gada 7. jūl. · Playing tag with terbium: New hydrophobic contacts and ligating amino acids are revealed in the 2.0-Å resolution X-ray crystal structure of a chemically evolved 17-residue lanthanide-binding peptide ...

Tīmeklislanthanide binding peptides. One such example is the EF hand motif which is a structural domain found in many calcium binding proteins and consists of two a-helices linked by a short calcium binding loop (Fig. 1).51 Several studies have shown that La3+ binds to the same EF domains that bind Ca2+.52–55 Indeed,

Tīmeklis2024. gada 4. aug. · Lanthanide complexes and methods of attaching them to … top urologists in northern virginiaTīmeklisHighlights: A mono-Eu-DTPA conjugated peptide ligand, Eu-DTPA-(A)-H2, has been developed. The choice of a site for incorporation of a chelator is critical. The labeled peptide retains full activity at the RXFP1 receptor. It is markedly cheaper to produce an top url shortenersTīmeklisThe creation of hydrolytically active Ln peptides that can fold, bind, cleave and … top urologist in yonkersTīmeklisThe investigation into the luminescence properties of a lanthanide-binding peptide, … top urrologist nyc by ratedTīmeklisNative EF-hand sequences bind to calcium or lanthanide ions via oxygen atoms … top urologists in houston txTīmeklis2024. gada 18. jūn. · Lanthanide complexes are used as convenient spectroscopic … top us acceleratorsTīmeklis2024. gada 14. apr. · Lanthanide complexes are used as convenient spectroscopic … top urologists in us