Tīmeklis2007. gada 6. jūn. · PMID: 17497862 DOI: 10.1021/ja070480v Abstract Lanthanide … Tīmeklis2024. gada 7. dec. · Brichtová, E.; Hudecová, J.; Vršková, N.; Šebestík, J.; Bouř, P.; …
Lanthanide-binding tags as versatile protein coexpression probes
Tīmeklis2016. gada 3. marts · LBTs have been broadly used as REE-binding fusion proteins for biological applications, including protein purification, biosensing, NMR spectroscopy, and X-ray crystallography.29–32The short peptide-based nature of LBTs and high REE binding affinity make them ideal for cell surface display to increase specific REE … TīmeklisThe separation of lanthanides from minor actinides such as americium and curium is an important step during the recycling process in the treatment of nuclear waste. However, the similar chemistry and ionic size of lanthanide and actinide ions make the separation challenging. Here, we report that a peptide-based reagent can selectively bind … top urologist in pune
Binding of Lanthanide Complexes to Histidine-Containing …
Tīmeklis2007. gada 12. maijs · A double-lanthanide-binding tag (dLBT), a small peptide sequence engineered to bind two lanthanide ions (e.g., Tb 3+) with high affinity, was used to solve the phase problem for the structure determination of ubiquitin by the single-wavelength anomalous diffraction (SAD) method. Tīmeklis2010. gada 1. apr. · Alternatively, peptide-based lanthanide-ion binding tags (LBTs) may be incorporated into proteins by standard molecular biology techniques, thereby avoiding steps that may be necessary for optimizing site-selective chemical modification [8].In this case, native protein sequences such as the Ca 2+-binding EF-hand … TīmeklisThe incorporation of unnatural chelating amino acids in short peptide sequences leads to lanthanide-binding peptides with a higher stability than sequences built exclusively from natural residues. In particular, the hexadentate peptide P22, which incorporates two unnatural amino acids Ada2 with aminodiacetate chelating arms, showed … top urologist in stuart florida