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Pcr4blunt topo

SpletComments for pCR®-Blunt II-TOPO® 3519 nucleotides lac promoter/operator region: bases 95-216 M13 Reverse priming site: bases 205-221 LacZ-alpha ORF: bases 217-576 SP6 promoter priming site: bases 239-256 Multiple Cloning Site: bases 269-399 TOPO®-Cloning site: bases 336-337 T7 promoter priming site: bases 406-425 SpletEine hocheffiziente, 5-minütige Klonierungsstrategie in einem Schritt („TOPO-Klonierung“) zum direkten Einfügen von mittels Proofreading-Polymerase-amplifizierten PCR-Produkten mit stumpfen Enden in einen Plasmidvektor für die Sequenzierung

Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™ …

SpletAnalyze Sequence: pCR blunt II TOPO. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest … SpletRACE PCR products can be quickly and easily cloned using either the Zero Blunt TOPO PCR Cloning Kit for Sequencing (blunt-end PCR products) or the TOPO TA Cloning for Sequencing Kit (PCR products with 3′ A-overhangs). Using the protocols provided, the cDNA ends of rare (30 copies/cell) and long (9 kb) transcripts can be amplified and ... counter column march instructions https://artworksvideo.com

Invitrogen™ Kit de clonage PCR Zero Blunt™ TOPO™, sans …

SpletpCR-BluntII-TOPO vector backbone. + Sequence information. + Datasheet. + Compare & Order pCR-BluntII-TOPO vector backbone products + TOP customer support. English +1 … SpletComments for pCR®-Blunt II-TOPO® 3519 nucleotides lac promoter/operator region: bases 95-216 M13 Reverse priming site: bases 205-221 LacZ-alpha ORF: bases 217-576 SP6 … SpletTOPO PCR Cloning reagents (Box 1) are listed below. Note that the user must supply the proofreading polymerase. Store Box 1 at −30°C to −10°C . Item Concentration Amount 10 … counter coffee filter holder

pCR-BluntII-TOPO-LINC00597(human)(有突变)-质粒载体-ATCC …

Category:pCR-BluntII-TOPO-DTWD2(human)_载体质粒_质粒载体网-质粒,微 …

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Pcr4blunt topo

pCR -Blunt II- TOPO - Thermo Fisher Scientific

Splet中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803 Splet质粒载体网zlzt.com专业提供pCR-BluntII-TOPO-GPR107(human),产品编号为zl-002714,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完美质粒众筹,质粒合成,质粒构建,质粒提取,质粒保藏!

Pcr4blunt topo

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SpletpCR4Blunt-TOPO (linearized) Linearized vector with bound topoisomerase, for TOPO® cloning of blunt PCR products. Can be used to generate nested deletions for sequencing. … Splet在 -80°C 下储存。一个单独的袋包含 10 个 S.N.A.P.™ 凝胶纯化柱，用于凝胶纯化 PCR 产物。在室温下储存。测序用 10 次反应 TOPO™ Cloning 试剂盒包含两盒。TOPO™ Cloning 盒储存在 -20°C 下。感受态大肠杆菌盒储存在 -70°C 下。妥善储存时，所有试剂均可保证 6 个 …

SpletZero Blunt TOPO PCR Cloning provides a highly efficient, 5 minute, one-step cloning strategy ("TOPO Cloning") for the direct insertion of blunt-end PCR products into a … SpletTOPO Cloning: Size: 3956: 5' Sequencing 1 Primer: T3: 5' Sequencing 1 Primer Sequence: T7: Stable: Unspecified: Constitutive: Unspecified: Viral/Non-Viral: Unspecified

Splet中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询 … SpletThe pCR4Blunt-TOPO vector used in this kit comes with no overhangs for efficient ligation of PCR products created by proofreading, thermostable polymerases that leave blunt-ended PCR products. The standard in cloning When it comes to cloning, TOPO cloning technology has been a reliable partner for thousands of scientists for over ten years.

SpletpTOP Blunt V2 (linearized) Linearized vector with bound topoisomerase, for efficient TOPO® cloning of blunt PCR products. Sequence Author: Enzynomics Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: TOPO Cloning Vectors More Plasmid Sets

SpletThe pCR™4Blunt-TOPO™ vector used in this kit comes with no overhangs for efficient ligation of PCR products created by proofreading, thermostable polymerases that leave … counter commander assault dutySpletZero Blunt® TOPO® PCR Cloning Kit for Sequencing . Five-minute cloning of blunt-end PCR products for sequencing . Catalog Numbers K2835-20, K2875-J10, K2875-20, K2875-40, … brenda the hate u giveSpletpCR®Blunt II-TOPO®ベクターの特徴： ポジティブセレクション用に ccd B遺伝子が搭載されています。 挿入配列を簡単に切り出すための、PCR生成物挿入部位に隣接したEcoRI … counter comicsSplet中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询 … brenda therapySplet质粒载体网zlzt.com专业提供pCR-BluntII-TOPO-DTWD2(human),产品编号为zl-000681,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完美质粒众筹, … counter combat strike csSpletpCR-BluntII-TOPO (Invitrogen, 450245) Zero Blunt TOPO PCR Cloning Kit. Map. brenda thelen wadena mnSpletThe pCR 4 Blunt-TOPO vector features: Contains the ccdB gene for positive selection; Short distance between sequencing primer sites and the insert site to as little as 33 bp. This … brenda the good witch