Webb12 juli 2013 · The numbers of the cell colonies were obtained and counted by 1-D gel quantity software QUANTITY ONE; (E) Cells were transfected with SNAT1-shRNA and sc-shRNA for 48 h. Then the cells were collected and the apoptosis rates were detected by flow cytometry; (F) After transfection for 48 h, 4T1 cells were harvested and cell cycle … Webb14 feb. 2014 · A vector containing a scrambled shRNA (sc-shRNA) served as a control. Specific knockdown of PKCδ by shRNA was growth-inhibitory in both the human prostate (PrCSC) and pancreatic (PCSC) cancer stem cells, with significant effects observed at early as 24 hr after infection, and progressing up to 72 hr (Figure 1A).
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Webb1.2.1 CIP2A shRNA的设计和构建 参考KHANNA等[7]在胃癌中的研究文献,选择5′-ACCATTGATATCCTTAGAA-3′作为干扰靶序列,结合shRNA设计原则,按上述靶位点设计编码具有发夹结构的siRNA正义链和反义链的表达载体,在每条寡核苷酸链两端分别加入用于酶切的Bam HⅠ和Hin dⅢ位点,以及作为RNA聚合酶Ⅲ转录终止信号 ... WebbshRNA SUPPORT REAGENTS shRNAPlasmid sc-108061 DeliversshRNAPlasmidDNAintocellswithminimalcell 0.2ml Transfection … gently used toys
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WebbM.Sc. 1996 Projects Epigenetic VEGF regulation in vivo Jan 2006 - Nov 2012. First publication to show epigenetic regulation of VEGF genes by lentiviral delivery of shRNA molecules. This resulted in therapeutics angiogenesis in ischemic mouse hindlimbs. See project. Languages ... Webb26 apr. 2016 · In exocytosis, soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE) proteins on secretory granules (SGs) and plasma membrane (PM) are assembled into a complex assisted by accessory proteins to hold SGs close to the PM ( 1 ). Webb14 apr. 2024 · 诱导性多能干细胞背后的秘密推手—Vitronectin蛋白. 近日,来自美国的Gameto公司宣布,他们将研发利用人类诱导性多功能干细胞(iPSCs)进行诱导生长的卵巢类器官模型-ovaroid。. 这一全人源卵巢类器官能够复制卵巢的许多关键功能,这标志着我们在女性生殖健康 ... gently used washing machines